Construct: shRNA TRCN0000020028

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016489.10-912s1c1
DNA Barcode:
CATTAGAAGTAGCCAACTCTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NT5C3A (51251)
Gene Description:
5'-nucleotidase, cytosolic IIIA
Transcript:
RefSeq NM_016489.10 (NON-CURRENT)
Match location:
Position 912 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000020028)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATTAGAAGTAGCCAACTCTA
Hairpin Sequence:
5'-CCGG-CATTAGAAGTAGCCAACTCTA-CTCGAG-TAGAGTTGGCTACTTCTAATG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATTAGAAGTAGCCAACTCTACTCGAGTAGAGTTGGCTACTTCTAATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATTAGAAGTAGCCAACTCTACTCGAGTAGAGTTGGCTACTTCTAATG-3'

Other clones with same target sequence:

(none)