Construct: shRNA TRCN0000020044

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_022114.2-3318s1c1
DNA Barcode:
GCCAATAGTGAGATGAACCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PRDM16 (63976)
Gene Description:
PR/SET domain 16
Transcript:
RefSeq NM_022114.2 (NON-CURRENT)
Match location:
Position 3318 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000020044)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCAATAGTGAGATGAACCAA
Hairpin Sequence:
5'-CCGG-GCCAATAGTGAGATGAACCAA-CTCGAG-TTGGTTCATCTCACTATTGGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCAATAGTGAGATGAACCAACTCGAGTTGGTTCATCTCACTATTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCAATAGTGAGATGAACCAACTCGAGTTGGTTCATCTCACTATTGGC-3'

Other clones with same target sequence:

(none)