Construct: shRNA TRCN0000020167

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005187.4-339s1c1
DNA Barcode:
CCCAGTGGACAGGAAAGCTAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CBFA2T3 (863)
Gene Description:
CBFA2/RUNX1 partner transcriptional co-repressor 3
Transcript:
RefSeq NM_005187.4 (NON-CURRENT)
Match location:
Position 339 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000020167)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCAGTGGACAGGAAAGCTAA
Hairpin Sequence:
5'-CCGG-CCCAGTGGACAGGAAAGCTAA-CTCGAG-TTAGCTTTCCTGTCCACTGGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCAGTGGACAGGAAAGCTAACTCGAGTTAGCTTTCCTGTCCACTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCAGTGGACAGGAAAGCTAACTCGAGTTAGCTTTCCTGTCCACTGGG-3'

Other clones with same target sequence:

(none)