Construct: shRNA TRCN0000020169

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003408.1-2333s1c1
DNA Barcode:
GCCATCTGTATAAGCCTCTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ZFP37 (7539)
Gene Description:
ZFP37 zinc finger protein
Transcript:
RefSeq NM_003408.1 (NON-CURRENT)
Match location:
Position 2333 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000020169)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCATCTGTATAAGCCTCTTT
Hairpin Sequence:
5'-CCGG-GCCATCTGTATAAGCCTCTTT-CTCGAG-AAAGAGGCTTATACAGATGGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCATCTGTATAAGCCTCTTTCTCGAGAAAGAGGCTTATACAGATGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCATCTGTATAAGCCTCTTTCTCGAGAAAGAGGCTTATACAGATGGC-3'

Other clones with same target sequence:

(none)