Construct: shRNA TRCN0000020223

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001239.2-1183s1c1
DNA Barcode:
CGACCTGGTAGAATCTCTCTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CCNH (902)
Gene Description:
cyclin H
Transcript:
RefSeq NM_001239.2 (NON-CURRENT)
Match location:
Position 1183 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000020223)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGACCTGGTAGAATCTCTCTA
Hairpin Sequence:
5'-CCGG-CGACCTGGTAGAATCTCTCTA-CTCGAG-TAGAGAGATTCTACCAGGTCG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGACCTGGTAGAATCTCTCTACTCGAGTAGAGAGATTCTACCAGGTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGACCTGGTAGAATCTCTCTACTCGAGTAGAGAGATTCTACCAGGTCG-3'

Other clones with same target sequence:

  1. TRCN0000342952