Construct: shRNA TRCN0000020334

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020781.2-2167s1c1
DNA Barcode:
CCCTCATAACTGGGCTTGAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ZNF398 (57541)
Gene Description:
zinc finger protein 398
Transcript:
RefSeq NM_020781.2 (NON-CURRENT)
Match location:
Position 2167 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000020334)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTCATAACTGGGCTTGAAA
Hairpin Sequence:
5'-CCGG-CCCTCATAACTGGGCTTGAAA-CTCGAG-TTTCAAGCCCAGTTATGAGGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTCATAACTGGGCTTGAAACTCGAGTTTCAAGCCCAGTTATGAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTCATAACTGGGCTTGAAACTCGAGTTTCAAGCCCAGTTATGAGGG-3'

Other clones with same target sequence:

(none)