Construct: shRNA TRCN0000020364

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018257.1-242s1c1
DNA Barcode:
GCAGACTATTATCTTGAAGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PCMTD2 (55251)
Gene Description:
protein-L-isoaspartate (D-aspartate) O-methyltransferase domain containing 2
Transcript:
RefSeq NM_018257.1 (NON-CURRENT)
Match location:
Position 242 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000020364)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGACTATTATCTTGAAGAA
Hairpin Sequence:
5'-CCGG-GCAGACTATTATCTTGAAGAA-CTCGAG-TTCTTCAAGATAATAGTCTGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGACTATTATCTTGAAGAACTCGAGTTCTTCAAGATAATAGTCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGACTATTATCTTGAAGAACTCGAGTTCTTCAAGATAATAGTCTGC-3'

Other clones with same target sequence:

(none)