Construct: shRNA TRCN0000020397

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020310.1-1823s1c1
DNA Barcode:
CCCGGCTACTGTCATGGCAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MNT (4335)
Gene Description:
MAX network transcriptional repressor
Transcript:
RefSeq NM_020310.1 (NON-CURRENT)
Match location:
Position 1823 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000020397)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCGGCTACTGTCATGGCAAA
Hairpin Sequence:
5'-CCGG-CCCGGCTACTGTCATGGCAAA-CTCGAG-TTTGCCATGACAGTAGCCGGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCGGCTACTGTCATGGCAAACTCGAGTTTGCCATGACAGTAGCCGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCGGCTACTGTCATGGCAAACTCGAGTTTGCCATGACAGTAGCCGGG-3'

Other clones with same target sequence:

(none)