Construct: shRNA TRCN0000020424

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002052.2-2922s1c1
DNA Barcode:
CCAGAGATTCTGCAACACGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GATA4 (2626)
Gene Description:
GATA binding protein 4
Transcript:
RefSeq NM_002052.2 (NON-CURRENT)
Match location:
Position 2922 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000020424)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGAGATTCTGCAACACGAA
Hairpin Sequence:
5'-CCGG-CCAGAGATTCTGCAACACGAA-CTCGAG-TTCGTGTTGCAGAATCTCTGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGAGATTCTGCAACACGAACTCGAGTTCGTGTTGCAGAATCTCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGAGATTCTGCAACACGAACTCGAGTTCGTGTTGCAGAATCTCTGG-3'

Other clones with same target sequence:

  1. TRCN0000329770