Construct: shRNA TRCN0000020478

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005962.2-647s1c1
DNA Barcode:
GCACACATCAAGAAACTTGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MXI1 (4601)
Gene Description:
MAX interactor 1, dimerization protein
Transcript:
RefSeq NM_005962.2 (NON-CURRENT)
Match location:
Position 647 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000020478)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACACATCAAGAAACTTGAA
Hairpin Sequence:
5'-CCGG-GCACACATCAAGAAACTTGAA-CTCGAG-TTCAAGTTTCTTGATGTGTGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACACATCAAGAAACTTGAACTCGAGTTCAAGTTTCTTGATGTGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACACATCAAGAAACTTGAACTCGAGTTCAAGTTTCTTGATGTGTGC-3'

Other clones with same target sequence:

  1. TRCN0000342878
  2. TRCN0000194207