Construct: shRNA TRCN0000020503

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005029.3-912s1c1
DNA Barcode:
CCTACGTCTATCGGGACCCGT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PITX3 (5309)
Gene Description:
paired like homeodomain 3
Transcript:
RefSeq NM_005029.3 (NON-CURRENT)
Match location:
Position 912 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000020503)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTACGTCTATCGGGACCCGT
Hairpin Sequence:
5'-CCGG-CCTACGTCTATCGGGACCCGT-CTCGAG-ACGGGTCCCGATAGACGTAGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTACGTCTATCGGGACCCGTCTCGAGACGGGTCCCGATAGACGTAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTACGTCTATCGGGACCCGTCTCGAGACGGGTCCCGATAGACGTAGG-3'

Other clones with same target sequence:

(none)