Construct: shRNA TRCN0000020526

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004571.3-242s1c1
DNA Barcode:
GCAAGTAGTAACAGAGTTAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PKNOX1 (5316)
Gene Description:
PBX/knotted 1 homeobox 1
Transcript:
RefSeq NM_004571.3 (NON-CURRENT)
Match location:
Position 242 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000020526)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAAGTAGTAACAGAGTTAAA
Hairpin Sequence:
5'-CCGG-GCAAGTAGTAACAGAGTTAAA-CTCGAG-TTTAACTCTGTTACTACTTGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAAGTAGTAACAGAGTTAAACTCGAGTTTAACTCTGTTACTACTTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAAGTAGTAACAGAGTTAAACTCGAGTTTAACTCTGTTACTACTTGC-3'

Other clones with same target sequence:

(none)