Construct: shRNA TRCN0000020681

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007122.2-588s1c1
DNA Barcode:
CACTGGTCAATTCTTTGTGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
USF1 (7391)
Gene Description:
upstream transcription factor 1
Transcript:
RefSeq NM_007122.2 (NON-CURRENT)
Match location:
Position 588 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000020681)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CACTGGTCAATTCTTTGTGAT
Hairpin Sequence:
5'-CCGG-CACTGGTCAATTCTTTGTGAT-CTCGAG-ATCACAAAGAATTGACCAGTG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCACTGGTCAATTCTTTGTGATCTCGAGATCACAAAGAATTGACCAGTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACACTGGTCAATTCTTTGTGATCTCGAGATCACAAAGAATTGACCAGTG-3'

Other clones with same target sequence:

(none)