Construct: shRNA TRCN0000020690

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_166527.6-3039s1c1
DNA Barcode:
GCGCCTCATTGAGCAAAGTAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
AP5Z1 (9907)
Gene Description:
adaptor related protein complex 5 subunit zeta 1
Transcript:
RefSeq XM_166527.6 (NON-CURRENT)
Match location:
Position 3039 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000020690)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGCCTCATTGAGCAAAGTAA
Hairpin Sequence:
5'-CCGG-GCGCCTCATTGAGCAAAGTAA-CTCGAG-TTACTTTGCTCAATGAGGCGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGCCTCATTGAGCAAAGTAACTCGAGTTACTTTGCTCAATGAGGCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGCCTCATTGAGCAAAGTAACTCGAGTTACTTTGCTCAATGAGGCGC-3'

Other clones with same target sequence:

(none)