Construct: shRNA TRCN0000020836
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_002096.1-1687s1c1
- DNA Barcode:
- GCGCAAGATGATCAACGACAA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- GTF2F1 (2962)
- Gene Description:
- general transcription factor IIF subunit 1
- Transcript:
- RefSeq NM_002096.1 (NON-CURRENT)
- Match location:
- Position 1687 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GCGCAAGATGATCAACGACAA
- Hairpin Sequence:
-
5'-CCGG-GCGCAAGATGATCAACGACAA-CTCGAG-TTGTCGTTGATCATCTTGCGC-TTTTT-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGCGCAAGATGATCAACGACAACTCGAGTTGTCGTTGATCATCTTGCGCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGCGCAAGATGATCAACGACAACTCGAGTTGTCGTTGATCATCTTGCGC-3'
Other clones with same target sequence:
-
TRCN0000318842
