Construct: shRNA TRCN0000020909

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_371617.2-1312s1c1
DNA Barcode:
GCTGACTCTTTCTACAAGATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CAND2 (23066)
Gene Description:
cullin associated and neddylation dissociated 2 (putative)
Transcript:
RefSeq XM_371617.2 (NON-CURRENT)
Match location:
Position 1312 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000020909)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGACTCTTTCTACAAGATT
Hairpin Sequence:
5'-CCGG-GCTGACTCTTTCTACAAGATT-CTCGAG-AATCTTGTAGAAAGAGTCAGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGACTCTTTCTACAAGATTCTCGAGAATCTTGTAGAAAGAGTCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGACTCTTTCTACAAGATTCTCGAGAATCTTGTAGAAAGAGTCAGC-3'

Other clones with same target sequence:

(none)