Construct: shRNA TRCN0000020964

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001515.2-1547s1c1
DNA Barcode:
GTCACAGAAATACCTGAAATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GTF2H2 (2966)
Gene Description:
general transcription factor IIH subunit 2
Transcript:
RefSeq NM_001515.2 (NON-CURRENT)
Match location:
Position 1547 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000020964)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTCACAGAAATACCTGAAATT
Hairpin Sequence:
5'-CCGG-GTCACAGAAATACCTGAAATT-CTCGAG-AATTTCAGGTATTTCTGTGAC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTCACAGAAATACCTGAAATTCTCGAGAATTTCAGGTATTTCTGTGACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTCACAGAAATACCTGAAATTCTCGAGAATTTCAGGTATTTCTGTGAC-3'

Other clones with same target sequence:

(none)