Construct: shRNA TRCN0000020994

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_376720.1-1701s1c1
DNA Barcode:
CCATGATAAATCATCTCGGTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SSPO (23145)
Gene Description:
SCO-spondin
Transcript:
RefSeq XM_376720.1 (NON-CURRENT)
Match location:
Position 1701 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000020994)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCATGATAAATCATCTCGGTT
Hairpin Sequence:
5'-CCGG-CCATGATAAATCATCTCGGTT-CTCGAG-AACCGAGATGATTTATCATGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCATGATAAATCATCTCGGTTCTCGAGAACCGAGATGATTTATCATGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCATGATAAATCATCTCGGTTCTCGAGAACCGAGATGATTTATCATGG-3'

Other clones with same target sequence:

(none)