Construct: shRNA TRCN0000021029

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_199244.1-959s1c1
DNA Barcode:
CGGATGCTTCTCTTTCAGCAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FOXD4L4 (349334)
Gene Description:
forkhead box D4 like 4
Transcript:
RefSeq NM_199244.1 (NON-CURRENT)
Match location:
Position 959 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000021029)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGATGCTTCTCTTTCAGCAT
Hairpin Sequence:
5'-CCGG-CGGATGCTTCTCTTTCAGCAT-CTCGAG-ATGCTGAAAGAGAAGCATCCG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGATGCTTCTCTTTCAGCATCTCGAGATGCTGAAAGAGAAGCATCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGATGCTTCTCTTTCAGCATCTCGAGATGCTGAAAGAGAAGCATCCG-3'

Other clones with same target sequence:

(none)