Construct: shRNA TRCN0000021127

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_032429.1-2058s1c1
DNA Barcode:
GATCACTGCTACTGAGATCTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LZTS2 (84445)
Gene Description:
leucine zipper tumor suppressor 2
Transcript:
RefSeq NM_032429.1 (NON-CURRENT)
Match location:
Position 2058 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000021127)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATCACTGCTACTGAGATCTA
Hairpin Sequence:
5'-CCGG-GATCACTGCTACTGAGATCTA-CTCGAG-TAGATCTCAGTAGCAGTGATC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATCACTGCTACTGAGATCTACTCGAGTAGATCTCAGTAGCAGTGATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATCACTGCTACTGAGATCTACTCGAGTAGATCTCAGTAGCAGTGATC-3'

Other clones with same target sequence:

(none)