Construct: shRNA TRCN0000021287

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000937.2-2516s1c1
DNA Barcode:
GCAGGACGTAATAGAGGTCAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
POLR2A (5430)
Gene Description:
RNA polymerase II subunit A
Transcript:
RefSeq NM_000937.2 (NON-CURRENT)
Match location:
Position 2516 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000021287)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGGACGTAATAGAGGTCAT
Hairpin Sequence:
5'-CCGG-GCAGGACGTAATAGAGGTCAT-CTCGAG-ATGACCTCTATTACGTCCTGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGGACGTAATAGAGGTCATCTCGAGATGACCTCTATTACGTCCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGGACGTAATAGAGGTCATCTCGAGATGACCTCTATTACGTCCTGC-3'

Other clones with same target sequence:

(none)