Construct: shRNA TRCN0000021291

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_032883.1-882s1c1
DNA Barcode:
ACTTTCGGTGACGTGTCCAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TOX2 (84969)
Gene Description:
TOX high mobility group box family member 2
Transcript:
RefSeq NM_032883.1 (NON-CURRENT)
Match location:
Position 882 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000021291)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACTTTCGGTGACGTGTCCAAA
Hairpin Sequence:
5'-CCGG-ACTTTCGGTGACGTGTCCAAA-CTCGAG-TTTGGACACGTCACCGAAAGT-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACTTTCGGTGACGTGTCCAAACTCGAGTTTGGACACGTCACCGAAAGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACTTTCGGTGACGTGTCCAAACTCGAGTTTGGACACGTCACCGAAAGT-3'

Other clones with same target sequence:

(none)