Construct: shRNA TRCN0000021292

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_032883.1-1568s1c1
DNA Barcode:
CAAATCGCTCTACCTCACCTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TOX2 (84969)
Gene Description:
TOX high mobility group box family member 2
Transcript:
RefSeq NM_032883.1 (NON-CURRENT)
Match location:
Position 1568 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000021292)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAAATCGCTCTACCTCACCTA
Hairpin Sequence:
5'-CCGG-CAAATCGCTCTACCTCACCTA-CTCGAG-TAGGTGAGGTAGAGCGATTTG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAAATCGCTCTACCTCACCTACTCGAGTAGGTGAGGTAGAGCGATTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAAATCGCTCTACCTCACCTACTCGAGTAGGTGAGGTAGAGCGATTTG-3'

Other clones with same target sequence:

(none)