Construct: shRNA TRCN0000021303

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_173825.1-586s1c1
DNA Barcode:
GCTGTCAAGCTCAGTAGGTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RABL3 (285282)
Gene Description:
RAB, member of RAS oncogene family like 3
Transcript:
RefSeq NM_173825.1 (NON-CURRENT)
Match location:
Position 586 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000021303)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGTCAAGCTCAGTAGGTTT
Hairpin Sequence:
5'-CCGG-GCTGTCAAGCTCAGTAGGTTT-CTCGAG-AAACCTACTGAGCTTGACAGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGTCAAGCTCAGTAGGTTTCTCGAGAAACCTACTGAGCTTGACAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGTCAAGCTCAGTAGGTTTCTCGAGAAACCTACTGAGCTTGACAGC-3'

Other clones with same target sequence:

(none)