Construct: shRNA TRCN0000021405

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_372199.2-1177s1c1
DNA Barcode:
CGGCTGAACTTCTGGTTAGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MAP3K15 (389840)
Gene Description:
mitogen-activated protein kinase kinase kinase 15
Transcript:
RefSeq XM_372199.2 (NON-CURRENT)
Match location:
Position 1177 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000021405)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGCTGAACTTCTGGTTAGAT
Hairpin Sequence:
5'-CCGG-CGGCTGAACTTCTGGTTAGAT-CTCGAG-ATCTAACCAGAAGTTCAGCCG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGCTGAACTTCTGGTTAGATCTCGAGATCTAACCAGAAGTTCAGCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGCTGAACTTCTGGTTAGATCTCGAGATCTAACCAGAAGTTCAGCCG-3'

Other clones with same target sequence:

(none)