Construct: shRNA TRCN0000021410

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_292160.5-333s1c1
DNA Barcode:
GCCTTCTTCAATTCATTTCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NEK5 (341676)
Gene Description:
NIMA related kinase 5
Transcript:
RefSeq XM_292160.5 (NON-CURRENT)
Match location:
Position 333 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000021410)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTTCTTCAATTCATTTCAA
Hairpin Sequence:
5'-CCGG-GCCTTCTTCAATTCATTTCAA-CTCGAG-TTGAAATGAATTGAAGAAGGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTTCTTCAATTCATTTCAACTCGAGTTGAAATGAATTGAAGAAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTTCTTCAATTCATTTCAACTCGAGTTGAAATGAATTGAAGAAGGC-3'

Other clones with same target sequence:

(none)