Construct: shRNA TRCN0000021418

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_114973.3-1544s1c1
DNA Barcode:
CGTGAATTACACCTTTGAAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
EPHA6 (203806)
Gene Description:
EPH receptor A6
Transcript:
RefSeq XM_114973.3 (NON-CURRENT)
Match location:
Position 1544 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000021418)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTGAATTACACCTTTGAAAT
Hairpin Sequence:
5'-CCGG-CGTGAATTACACCTTTGAAAT-CTCGAG-ATTTCAAAGGTGTAATTCACG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTGAATTACACCTTTGAAATCTCGAGATTTCAAAGGTGTAATTCACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTGAATTACACCTTTGAAATCTCGAGATTTCAAAGGTGTAATTCACG-3'

Other clones with same target sequence:

(none)