Construct: shRNA TRCN0000021449

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_291141.3-1801s1c1
DNA Barcode:
GCCCAGGATCTGATTACCTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MAST4 (23227)
Gene Description:
microtubule associated serine/threonine kinase family member 4
Transcript:
RefSeq XM_291141.3 (NON-CURRENT)
Match location:
Position 1801 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000021449)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCCAGGATCTGATTACCTTA
Hairpin Sequence:
5'-CCGG-GCCCAGGATCTGATTACCTTA-CTCGAG-TAAGGTAATCAGATCCTGGGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCCAGGATCTGATTACCTTACTCGAGTAAGGTAATCAGATCCTGGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCCAGGATCTGATTACCTTACTCGAGTAAGGTAATCAGATCCTGGGC-3'

Other clones with same target sequence:

(none)