Construct: shRNA TRCN0000021472

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_290516.5-2903s1c1
DNA Barcode:
CACGCATCTTTAGGGTGACAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CDC42BPG (55561)
Gene Description:
CDC42 binding protein kinase gamma
Transcript:
RefSeq XM_290516.5 (NON-CURRENT)
Match location:
Position 2903 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000021472)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CACGCATCTTTAGGGTGACAA
Hairpin Sequence:
5'-CCGG-CACGCATCTTTAGGGTGACAA-CTCGAG-TTGTCACCCTAAAGATGCGTG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCACGCATCTTTAGGGTGACAACTCGAGTTGTCACCCTAAAGATGCGTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACACGCATCTTTAGGGTGACAACTCGAGTTGTCACCCTAAAGATGCGTG-3'

Other clones with same target sequence:

(none)