Construct: shRNA TRCN0000021484

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_055866.5-4745s1c1
DNA Barcode:
CGGAACTGAACTGAACTCTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LMTK3 (114783)
Gene Description:
lemur tyrosine kinase 3
Transcript:
RefSeq XM_055866.5 (NON-CURRENT)
Match location:
Position 4745 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000021484)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGAACTGAACTGAACTCTTT
Hairpin Sequence:
5'-CCGG-CGGAACTGAACTGAACTCTTT-CTCGAG-AAAGAGTTCAGTTCAGTTCCG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGAACTGAACTGAACTCTTTCTCGAGAAAGAGTTCAGTTCAGTTCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGAACTGAACTGAACTCTTTCTCGAGAAAGAGTTCAGTTCAGTTCCG-3'

Other clones with same target sequence:

(none)