Construct: shRNA TRCN0000021507

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020247.3-966s1c1
DNA Barcode:
GCATCCAGGATGATGCCTTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
COQ8A (56997)
Gene Description:
coenzyme Q8A
Transcript:
RefSeq NM_020247.3 (NON-CURRENT)
Match location:
Position 966 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000021507)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCATCCAGGATGATGCCTTTA
Hairpin Sequence:
5'-CCGG-GCATCCAGGATGATGCCTTTA-CTCGAG-TAAAGGCATCATCCTGGATGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCATCCAGGATGATGCCTTTACTCGAGTAAAGGCATCATCCTGGATGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCATCCAGGATGATGCCTTTACTCGAGTAAAGGCATCATCCTGGATGC-3'

Other clones with same target sequence:

(none)