Construct: shRNA TRCN0000021516

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003942.1-521s1c1
DNA Barcode:
GCACAAGCTCGGCATCATTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RPS6KA4 (8986)
Gene Description:
ribosomal protein S6 kinase A4
Transcript:
RefSeq NM_003942.1 (NON-CURRENT)
Match location:
Position 521 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000021516)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACAAGCTCGGCATCATTTA
Hairpin Sequence:
5'-CCGG-GCACAAGCTCGGCATCATTTA-CTCGAG-TAAATGATGCCGAGCTTGTGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACAAGCTCGGCATCATTTACTCGAGTAAATGATGCCGAGCTTGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACAAGCTCGGCATCATTTACTCGAGTAAATGATGCCGAGCTTGTGC-3'

Other clones with same target sequence:

  1. TRCN0000279790