Construct: shRNA TRCN0000021519

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_293029.3-970s1c1
DNA Barcode:
GCTATAAAAATAATTCCTTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CDKL4 (344387)
Gene Description:
cyclin dependent kinase like 4
Transcript:
RefSeq XM_293029.3 (NON-CURRENT)
Match location:
Position 970 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000021519)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTATAAAAATAATTCCTTTT
Hairpin Sequence:
5'-CCGG-GCTATAAAAATAATTCCTTTT-CTCGAG-AAAAGGAATTATTTTTATAGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTATAAAAATAATTCCTTTTCTCGAGAAAAGGAATTATTTTTATAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTATAAAAATAATTCCTTTTCTCGAGAAAAGGAATTATTTTTATAGC-3'

Other clones with same target sequence:

(none)