Construct: shRNA TRCN0000021542
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- XM_032901.7-60s1c1
- DNA Barcode:
- CGGTGTTCACTACCCTAATAA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- RUBCN (9711)
- Gene Description:
- rubicon autophagy regulator
- Transcript:
- RefSeq XM_032901.7 (NON-CURRENT)
- Match location:
- Position 60 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000021542)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- CGGTGTTCACTACCCTAATAA
- Hairpin Sequence:
-
5'-CCGG-CGGTGTTCACTACCCTAATAA-CTCGAG-TTATTAGGGTAGTGAACACCG-TTTTT-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGCGGTGTTCACTACCCTAATAACTCGAGTTATTAGGGTAGTGAACACCGTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAACGGTGTTCACTACCCTAATAACTCGAGTTATTAGGGTAGTGAACACCG-3'
Other clones with same target sequence:
(none)
