Construct: shRNA TRCN0000021563

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_017672.2-504s1c1
DNA Barcode:
CGGATGCTTATGGAGTCATAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TRPM7 (54822)
Gene Description:
transient receptor potential cation channel subfamily M member 7
Transcript:
RefSeq NM_017672.2 (NON-CURRENT)
Match location:
Position 504 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000021563)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGATGCTTATGGAGTCATAA
Hairpin Sequence:
5'-CCGG-CGGATGCTTATGGAGTCATAA-CTCGAG-TTATGACTCCATAAGCATCCG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGATGCTTATGGAGTCATAACTCGAGTTATGACTCCATAAGCATCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGATGCTTATGGAGTCATAACTCGAGTTATGACTCCATAAGCATCCG-3'

Other clones with same target sequence:

(none)