Construct: shRNA TRCN0000021583

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_012224.1-3581s1c1
DNA Barcode:
CCTTGCTGATTGGACTTTCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NEK1 (4750)
Gene Description:
NIMA related kinase 1
Transcript:
RefSeq NM_012224.1 (NON-CURRENT)
Match location:
Position 3581 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000021583)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTGCTGATTGGACTTTCAA
Hairpin Sequence:
5'-CCGG-CCTTGCTGATTGGACTTTCAA-CTCGAG-TTGAAAGTCCAATCAGCAAGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTGCTGATTGGACTTTCAACTCGAGTTGAAAGTCCAATCAGCAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTGCTGATTGGACTTTCAACTCGAGTTGAAAGTCCAATCAGCAAGG-3'

Other clones with same target sequence:

(none)