Construct: shRNA TRCN0000021587

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_017662.3-1336s1c1
DNA Barcode:
CCAAATTCTAATGGAGTGTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TRPM6 (140803)
Gene Description:
transient receptor potential cation channel subfamily M member 6
Transcript:
RefSeq NM_017662.3 (NON-CURRENT)
Match location:
Position 1336 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000021587)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAAATTCTAATGGAGTGTAT
Hairpin Sequence:
5'-CCGG-CCAAATTCTAATGGAGTGTAT-CTCGAG-ATACACTCCATTAGAATTTGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAAATTCTAATGGAGTGTATCTCGAGATACACTCCATTAGAATTTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAAATTCTAATGGAGTGTATCTCGAGATACACTCCATTAGAATTTGG-3'

Other clones with same target sequence:

(none)