Construct: shRNA TRCN0000021599

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_052843.1-20060s1c1
DNA Barcode:
CCTTCTTACCTCACTCAACTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
OBSCN (84033)
Gene Description:
obscurin, cytoskeletal calmodulin and titin-interacting RhoGEF
Transcript:
RefSeq NM_052843.1 (NON-CURRENT)
Match location:
Position 20060 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000021599)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTCTTACCTCACTCAACTT
Hairpin Sequence:
5'-CCGG-CCTTCTTACCTCACTCAACTT-CTCGAG-AAGTTGAGTGAGGTAAGAAGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTCTTACCTCACTCAACTTCTCGAGAAGTTGAGTGAGGTAAGAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTCTTACCTCACTCAACTTCTCGAGAAGTTGAGTGAGGTAAGAAGG-3'

Other clones with same target sequence:

(none)