Construct: shRNA TRCN0000021620

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003889.2-2682s1c1
DNA Barcode:
CGAGCTGTGTCAACTGAGATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NR1I2 (8856)
Gene Description:
nuclear receptor subfamily 1 group I member 2
Transcript:
RefSeq NM_003889.2 (NON-CURRENT)
Match location:
Position 2682 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000021620)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGAGCTGTGTCAACTGAGATT
Hairpin Sequence:
5'-CCGG-CGAGCTGTGTCAACTGAGATT-CTCGAG-AATCTCAGTTGACACAGCTCG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGAGCTGTGTCAACTGAGATTCTCGAGAATCTCAGTTGACACAGCTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGAGCTGTGTCAACTGAGATTCTCGAGAATCTCAGTTGACACAGCTCG-3'

Other clones with same target sequence:

(none)