Construct: shRNA TRCN0000021630

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007067.3-805s1c1
DNA Barcode:
CGGGATAAGCAGATAGAAGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
KAT7 (11143)
Gene Description:
lysine acetyltransferase 7
Transcript:
RefSeq NM_007067.3 (NON-CURRENT)
Match location:
Position 805 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000021630)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGGATAAGCAGATAGAAGAA
Hairpin Sequence:
5'-CCGG-CGGGATAAGCAGATAGAAGAA-CTCGAG-TTCTTCTATCTGCTTATCCCG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGGATAAGCAGATAGAAGAACTCGAGTTCTTCTATCTGCTTATCCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGGATAAGCAGATAGAAGAACTCGAGTTCTTCTATCTGCTTATCCCG-3'

Other clones with same target sequence:

(none)