Construct: shRNA TRCN0000021670

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003269.2-1804s1c1
DNA Barcode:
CCATCGGCAATGTGCCAATTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NR2E1 (7101)
Gene Description:
nuclear receptor subfamily 2 group E member 1
Transcript:
RefSeq NM_003269.2 (NON-CURRENT)
Match location:
Position 1804 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000021670)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCATCGGCAATGTGCCAATTA
Hairpin Sequence:
5'-CCGG-CCATCGGCAATGTGCCAATTA-CTCGAG-TAATTGGCACATTGCCGATGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCATCGGCAATGTGCCAATTACTCGAGTAATTGGCACATTGCCGATGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCATCGGCAATGTGCCAATTACTCGAGTAATTGGCACATTGCCGATGG-3'

Other clones with same target sequence:

(none)