Construct: shRNA TRCN0000021679

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006235.1-827s1c1
DNA Barcode:
TGGACACCTTACACCGAGTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
POU2AF1 (5450)
Gene Description:
POU class 2 homeobox associating factor 1
Transcript:
RefSeq NM_006235.1 (NON-CURRENT)
Match location:
Position 827 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000021679)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGGACACCTTACACCGAGTAT
Hairpin Sequence:
5'-CCGG-TGGACACCTTACACCGAGTAT-CTCGAG-ATACTCGGTGTAAGGTGTCCA-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGGACACCTTACACCGAGTATCTCGAGATACTCGGTGTAAGGTGTCCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGGACACCTTACACCGAGTATCTCGAGATACTCGGTGTAAGGTGTCCA-3'

Other clones with same target sequence:

(none)