Construct: shRNA TRCN0000021690

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003147.4-639s1c1
DNA Barcode:
CACACACAACATTGGTCGATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SSX2 (6757)
Gene Description:
SSX family member 2
Transcript:
RefSeq NM_003147.4 (NON-CURRENT)
Match location:
Position 639 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000021690)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CACACACAACATTGGTCGATT
Hairpin Sequence:
5'-CCGG-CACACACAACATTGGTCGATT-CTCGAG-AATCGACCAATGTTGTGTGTG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCACACACAACATTGGTCGATTCTCGAGAATCGACCAATGTTGTGTGTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACACACACAACATTGGTCGATTCTCGAGAATCGACCAATGTTGTGTGTG-3'

Other clones with same target sequence:

(none)