Construct: shRNA TRCN0000021704

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_031283.1-1320s1c1
DNA Barcode:
CGGGACAACTATGGTAAGAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TCF7L1 (83439)
Gene Description:
transcription factor 7 like 1
Transcript:
RefSeq NM_031283.1 (NON-CURRENT)
Match location:
Position 1320 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000021704)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGGACAACTATGGTAAGAAA
Hairpin Sequence:
5'-CCGG-CGGGACAACTATGGTAAGAAA-CTCGAG-TTTCTTACCATAGTTGTCCCG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGGACAACTATGGTAAGAAACTCGAGTTTCTTACCATAGTTGTCCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGGACAACTATGGTAAGAAACTCGAGTTTCTTACCATAGTTGTCCCG-3'

Other clones with same target sequence:

(none)