Construct: shRNA TRCN0000021715

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000238.2-91s1c1
DNA Barcode:
CCGTAAGTTCATCATCGCCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
KCNH2 (3757)
Gene Description:
potassium voltage-gated channel subfamily H member 2
Transcript:
RefSeq NM_000238.2 (NON-CURRENT)
Match location:
Position 91 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000021715)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGTAAGTTCATCATCGCCAA
Hairpin Sequence:
5'-CCGG-CCGTAAGTTCATCATCGCCAA-CTCGAG-TTGGCGATGATGAACTTACGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGTAAGTTCATCATCGCCAACTCGAGTTGGCGATGATGAACTTACGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGTAAGTTCATCATCGCCAACTCGAGTTGGCGATGATGAACTTACGG-3'

Other clones with same target sequence:

(none)