Construct: shRNA TRCN0000021740

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005654.3-1321s1c1
DNA Barcode:
CAGCTTCAACTGGCCTTACAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NR2F1 (7025)
Gene Description:
nuclear receptor subfamily 2 group F member 1
Transcript:
RefSeq NM_005654.3 (NON-CURRENT)
Match location:
Position 1321 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000021740)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGCTTCAACTGGCCTTACAT
Hairpin Sequence:
5'-CCGG-CAGCTTCAACTGGCCTTACAT-CTCGAG-ATGTAAGGCCAGTTGAAGCTG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGCTTCAACTGGCCTTACATCTCGAGATGTAAGGCCAGTTGAAGCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGCTTCAACTGGCCTTACATCTCGAGATGTAAGGCCAGTTGAAGCTG-3'

Other clones with same target sequence:

  1. TRCN0000322965