Construct: shRNA TRCN0000021761

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005636.3-259s1c1
DNA Barcode:
CCCACCTTTCATGCGTAGTAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SSX4 (6759)
Gene Description:
SSX family member 4
Transcript:
RefSeq NM_005636.3 (NON-CURRENT)
Match location:
Position 259 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000021761)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCACCTTTCATGCGTAGTAA
Hairpin Sequence:
5'-CCGG-CCCACCTTTCATGCGTAGTAA-CTCGAG-TTACTACGCATGAAAGGTGGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCACCTTTCATGCGTAGTAACTCGAGTTACTACGCATGAAAGGTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCACCTTTCATGCGTAGTAACTCGAGTTACTACGCATGAAAGGTGGG-3'

Other clones with same target sequence:

(none)