Construct: shRNA TRCN0000021798

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015557.1-1079s1c1
DNA Barcode:
CGCAAGAAGAAGAGGATTGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CHD5 (26038)
Gene Description:
chromodomain helicase DNA binding protein 5
Transcript:
RefSeq NM_015557.1 (NON-CURRENT)
Match location:
Position 1079 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000021798)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCAAGAAGAAGAGGATTGAT
Hairpin Sequence:
5'-CCGG-CGCAAGAAGAAGAGGATTGAT-CTCGAG-ATCAATCCTCTTCTTCTTGCG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCAAGAAGAAGAGGATTGATCTCGAGATCAATCCTCTTCTTCTTGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCAAGAAGAAGAGGATTGATCTCGAGATCAATCCTCTTCTTCTTGCG-3'

Other clones with same target sequence:

(none)