Construct: shRNA TRCN0000021802

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024674.3-679s1c1
DNA Barcode:
ACCTACTTTCGAGAGGAAGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LIN28A (79727)
Gene Description:
lin-28 homolog A
Transcript:
RefSeq NM_024674.3 (NON-CURRENT)
Match location:
Position 679 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000021802)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACCTACTTTCGAGAGGAAGAA
Hairpin Sequence:
5'-CCGG-ACCTACTTTCGAGAGGAAGAA-CTCGAG-TTCTTCCTCTCGAAAGTAGGT-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACCTACTTTCGAGAGGAAGAACTCGAGTTCTTCCTCTCGAAAGTAGGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACCTACTTTCGAGAGGAAGAACTCGAGTTCTTCCTCTCGAAAGTAGGT-3'

Other clones with same target sequence:

(none)