Construct: shRNA TRCN0000021809

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_012236.1-1411s1c1
DNA Barcode:
CCCAACAGTTTGTATCTACTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SCMH1 (22955)
Gene Description:
Scm polycomb group protein homolog 1
Transcript:
RefSeq NM_012236.1 (NON-CURRENT)
Match location:
Position 1411 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000021809)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCAACAGTTTGTATCTACTT
Hairpin Sequence:
5'-CCGG-CCCAACAGTTTGTATCTACTT-CTCGAG-AAGTAGATACAAACTGTTGGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCAACAGTTTGTATCTACTTCTCGAGAAGTAGATACAAACTGTTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCAACAGTTTGTATCTACTTCTCGAGAAGTAGATACAAACTGTTGGG-3'

Other clones with same target sequence:

(none)